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At least 30 people were killed in an Israeli strike near an aid distribution center operated by the Gaza Humanitarian Foundation (GHF) in Rafah, sources reported on Sunday citing Palestinian news agency WAFA and Hamas-affiliated media.
The incident also reportedly left more than 115 people injured.
The Israeli government has not issued an official statement regarding the reported attack.
The aid site is run by the GHF, a group that is supported by both international donors and Israel. The GHF recently began operating in Gaza.
According to Reuters, while some Palestinians have questioned GHF's neutrality and raised concerns about Israel using biometric and other checks, Israeli officials said the checks are used to make sure no one connected to Hamas receives aid.
On May 28, Hamas accused Israel of killing at least three people and injuring 46 near a GHF distribution site. GHF denied the claim.
The Israeli military said its forces fired warning shots near the compound to regain control after thousands of people gathered at the site.