Ecuador will take on Senegal at the Khalifa International Stadium at 8.30 PM Indian Standard Time.
The South American team is in the second spot and anything apart from a loss would see them make it to the next round.
Senegal will be aiming to bag all 3 points as that will make it easier for them to finish in the top two.
In the second match, the Netherlands will take on Qatar at the Al-Bayt Stadium.
In Group A, the Netherlands is leading with four points whereas Qatar is yet to open their account.
Sources reports, yesterday Portugal entered the last 16 stage, beating Uruguay 2-0.
Bruno Fernandes scored twice to send Portugal flying into the Round of 16, defeating Uruguay 2-0.
Fernandes, curled in a cross from the left that barely went over the head of Ronaldo and bounced into the far corner of the net in the 54th minute to give Portugal a 1-0 lead.
Ronaldo wheeled away in celebration, believing he had scored his ninth World Cup goal and second of this year's tournament.
However, after repeated close-up replays, it was awarded to Fernandes, who added a second goal from the penalty spot in stoppage time after a handball by Jose Maria Gimenez.
Earlier, in Brazil versus Switzerland, after playing a goalless first half at Stadium 974, Manchester United star Casemiro scored a stunning volley to give Brazil, playing without Neymar, a 1-0 win over Switzerland.
With the thrilling win in a low-scoring encounter, the record five-time champions have joined France in the last 16 of the Qatar World Cup.
The Brazilian side has also become the second team to not face a single shot on target in their first two matches in a single edition of the FIFA World Cup.
The same feat earlier was achieved by defending champions France in the 1998 edition of the mega event.